Fast tunable ultra-violet laser source for confocal fura-2AM imaging
نویسندگان
چکیده
We report upon a novel wavelength-flexible laser source for three-dimensional ultraviolet imaging. Based on supercontinuum generation in photonic crystal fiber, the resultant broadband source extended from =331 nm into the visible region of the spectrum. Using an electronically-controlled filter wheel and filter set with a response time of approximately 50 ms, rapid wavelength selection was performed. The described scheme is capable of exciting the current range of ultra-violet-excited fluorophores and the simple and rapid wavelength control also provides a new approach for fast ratiometric imaging of Fura-2AM, facilitating an easy method of performing quantitative intracellular calcium concentration measurements.
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